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rabbit anti sars cov 2 spike s2 domain (Thermo Fisher)

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Thermo Fisher rabbit anti sars cov 2 spike s2 domain
Rabbit Anti Sars Cov 2 Spike S2 Domain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+sars+cov+2+spike+s2+domain/SUCROSE+EP%2FBP%2FNF+12KG/pm39753670-461-13-18
Average 99 stars, based on 1 article reviews

rabbit anti sars cov 2 spike s2 domain - by Bioz Stars, 2026-11

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Article Title: Phenotypic evolution of SARS-CoV-2 spike during the COVID-19 pandemic.
Article Snippet: Viral RNA was extracted using a QIAamp viral RNA mini kit (Qiagen, 52906) and viral genome sequenced using Oxford Nanopore as described below91.

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Article Title: Phenotypic evolution of SARS-CoV-2 spike during the COVID-19 pandemic
Article Snippet: Cells and purified virus particles on beads were lysed in ice-cold lysis buffer containing 50 mM Tris/HCl pH 7.5, 1 mM EGTA, 1 mM EDTA, 1% (v/v) Triton X-100, 1 mM sodium orthovanadate, 50 mM sodium fluoride, 5 mM sodium pyrophosphate, 0.27 M sucrose, 10 mM sodium 2-glycerophosphate, 1 mM phenylmethylsulphonyl fluoride, 1 mM benzamidine and 1× NuPAGE LDS sample buffer (NP0007, ThermoFisher). .. Samples were then analysed by SDS–PAGE and immunoblotting using rabbit anti-SARS-CoV-2 spike S2 domain (ThermoFisher, PA1-41165) and according to the assay, rabbit anti-phospho STAT1 Tyr701 clone 58D6 (Cell Signaling Technologies, 9167), rabbit anti-RSAD2 (Proteintech, 28089-1-AP), mouse anti-IFIT-1 (Origene, TA5009487), mouse anti-alpha-tubulin DM1A (Sigma Aldrich, T6199) and sheep anti-SARS-CoV-2 nucleocapsid protein (3rd bleed) . .. Secondary antibodies anti-rabbit IgG (H + L) DyLight 800 conjugate (Cell Signalling Technology, 5151S), anti-mouse IgG (H + L) DyLight 680 conjugate (Cell Signalling Technology, 5470S) and donkey anti-sheep IgG451 (H + L) DyLight 800 (ThermoFisher, SA5-10060) were used for immunoblotting before protein visualization using the Odyssey CLx imager (Li-Cor).

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SARS-CoV-2 A.23.1 S sequence changes and S1/S2 furin cleavage. (A) Summary of notable functional domains and sequence changes in the spike gene of A.23.1 compared to Wuhan-Hu-1 and B.1.617.2 (Delta). (B) Furin cleavage score analysis of CoV S1/S2 cleavage sites. CoV S sequences were analyzed using the ProP1 1.0 and PiTou2 3.0 furin prediction algorithm, generating a score with bold numbers indicating predicted furin cleavage. | denotes the position of the predicted S1/S2 cleavage site. Basic resides, arginine (R) and lysine (K), are highlighted in blue, with histidine in purple. Sequences corresponding to the S1/S2 region of SARS-CoV-2 ( QHD43416.1 ), SARS-CoV ( AAT74874.1 ), MERS-CoV ( AFS88936.1 ), HCoV-HKU1 ( AAT98580.1 ), and HCoV-OC43 ( KY369907.1 ) were obtained from GenBank. Sequences corresponding to the S1/S2 region of SARS-CoV-2 B.1.1.7 (EPI_ISL_1374509) and SARS-CoV-2 A.23.1 hCoV-19/Uganda/UG185/2020 (EPI_ISL_955136), were obtained from GISAID. (C) Fluorogenic peptide cleavage assays of the SARS-CoV-2 S1/S2 cleavage site. Peptides mimicking the S1/S2 site of the SARS-CoV-2 Wuhan-Hu-1 (WT – P681), B.1.1.7 (P681H), and A.23.1 (P681R) variants were evaluated for in vitro cleavage by furin, compared to trypsin control. Error bars represent G standard errors ( n = 9). Asterisks indicate statistical significance compared to the untreated control. Statistical analysis was performed using an unpaired Student's t test. **, P < 0.01.

Journal: Microbiology Spectrum

Article Title: Spike Protein Cleavage-Activation in the Context of the SARS-CoV-2 P681R Mutation: an Analysis from Its First Appearance in Lineage A.23.1 Identified in Uganda

doi: 10.1128/spectrum.01514-22

Figure Lengend Snippet: SARS-CoV-2 A.23.1 S sequence changes and S1/S2 furin cleavage. (A) Summary of notable functional domains and sequence changes in the spike gene of A.23.1 compared to Wuhan-Hu-1 and B.1.617.2 (Delta). (B) Furin cleavage score analysis of CoV S1/S2 cleavage sites. CoV S sequences were analyzed using the ProP1 1.0 and PiTou2 3.0 furin prediction algorithm, generating a score with bold numbers indicating predicted furin cleavage. | denotes the position of the predicted S1/S2 cleavage site. Basic resides, arginine (R) and lysine (K), are highlighted in blue, with histidine in purple. Sequences corresponding to the S1/S2 region of SARS-CoV-2 ( QHD43416.1 ), SARS-CoV ( AAT74874.1 ), MERS-CoV ( AFS88936.1 ), HCoV-HKU1 ( AAT98580.1 ), and HCoV-OC43 ( KY369907.1 ) were obtained from GenBank. Sequences corresponding to the S1/S2 region of SARS-CoV-2 B.1.1.7 (EPI_ISL_1374509) and SARS-CoV-2 A.23.1 hCoV-19/Uganda/UG185/2020 (EPI_ISL_955136), were obtained from GISAID. (C) Fluorogenic peptide cleavage assays of the SARS-CoV-2 S1/S2 cleavage site. Peptides mimicking the S1/S2 site of the SARS-CoV-2 Wuhan-Hu-1 (WT – P681), B.1.1.7 (P681H), and A.23.1 (P681R) variants were evaluated for in vitro cleavage by furin, compared to trypsin control. Error bars represent G standard errors ( n = 9). Asterisks indicate statistical significance compared to the untreated control. Statistical analysis was performed using an unpaired Student's t test. **, P < 0.01.

Article Snippet: SARS-CoV-2 S was detected using a rabbit polyclonal antibody against the S2 domain (cat.: 40590-T62, Sinobiological).

Techniques: Sequencing, Functional Assay, In Vitro

Cell-to-cell fusion in SARS-CoV-2 A.23.1 S expressing cells. (A) Cell-to-cell fusion assay of SARS-CoV-2 Wuhan-Hu-1 S (WT), SARS-CoV-2 S A.23.1 variant, or SARS-CoV-2 S WT with P681R mutation. S-, non-transfected cells. SARS-CoV-2 S was detected using a rabbit antibody against the SARS-CoV-2 S2 region. (B) Syncytia quantification by number of nuclei involved in syncytia. (C) Western blot analysis of membrane expressed S proteins and GLUT4 (housekeeping expression protein). All of the experiments were performed on Vero E6 and Vero-TMPRSS2 cells.

Journal: Microbiology Spectrum

Article Title: Spike Protein Cleavage-Activation in the Context of the SARS-CoV-2 P681R Mutation: an Analysis from Its First Appearance in Lineage A.23.1 Identified in Uganda

doi: 10.1128/spectrum.01514-22

Figure Lengend Snippet: Cell-to-cell fusion in SARS-CoV-2 A.23.1 S expressing cells. (A) Cell-to-cell fusion assay of SARS-CoV-2 Wuhan-Hu-1 S (WT), SARS-CoV-2 S A.23.1 variant, or SARS-CoV-2 S WT with P681R mutation. S-, non-transfected cells. SARS-CoV-2 S was detected using a rabbit antibody against the SARS-CoV-2 S2 region. (B) Syncytia quantification by number of nuclei involved in syncytia. (C) Western blot analysis of membrane expressed S proteins and GLUT4 (housekeeping expression protein). All of the experiments were performed on Vero E6 and Vero-TMPRSS2 cells.

Article Snippet: SARS-CoV-2 S was detected using a rabbit polyclonal antibody against the S2 domain (cat.: 40590-T62, Sinobiological).

Techniques: Expressing, Cell Fusion Assay, Variant Assay, Mutagenesis, Transfection, Western Blot

SARS-CoV-2 A.23.1 variant S1/S2 cleavage site activation and role in viral entry. (A) Western blot analysis of MLVpp-SARS-CoV-2 S produced in ± dec-RVKR-CMK and treated with ± furin. S was detected using a rabbit antibody against the SARS-CoV-2 S2 subunit. MLV content was detected using a mouse antibody against MLV p30. (B) Pseudoparticle infectivity assays in Vero E6 and Vero-TMPRSS2 cells. Cells were infected with MLVpps harboring the SARS-CoV-2 S (WT), SARS-CoV-2 S A.23.1 variant, and SARS-CoV-2 S WT with P681R mutation. Data represent the average luciferase activity of cells of four independent experiments (Vero E6 and Vero-TMPRSS2). Error bars represent G standard deviation ( n = 4). Asterisks indicate statistical significance compared to the untreated control. Statistical analysis was performed using an unpaired Student's t test. *, P < 0.1; ****, P < 0.0001. (C) Pseudoparticle infectivity assays in A549-ACE2-TMPRSS2 cells. Cells were infected with MLVpps harboring the SARS-CoV-2 S (WT), SARS-CoV-2 S A.23.1 variant, and SARS-CoV-2 S WT with P681R mutation. Data represent the average luciferase activity of cells of three independent experiments. Error bars represent G standard deviation ( n = 3). Asterisks indicate statistical significance compared to the untreated control. Statistical analysis was performed using an unpaired Student's t test. ****, P < 0.0001.

Journal: Microbiology Spectrum

Article Title: Spike Protein Cleavage-Activation in the Context of the SARS-CoV-2 P681R Mutation: an Analysis from Its First Appearance in Lineage A.23.1 Identified in Uganda

doi: 10.1128/spectrum.01514-22

Figure Lengend Snippet: SARS-CoV-2 A.23.1 variant S1/S2 cleavage site activation and role in viral entry. (A) Western blot analysis of MLVpp-SARS-CoV-2 S produced in ± dec-RVKR-CMK and treated with ± furin. S was detected using a rabbit antibody against the SARS-CoV-2 S2 subunit. MLV content was detected using a mouse antibody against MLV p30. (B) Pseudoparticle infectivity assays in Vero E6 and Vero-TMPRSS2 cells. Cells were infected with MLVpps harboring the SARS-CoV-2 S (WT), SARS-CoV-2 S A.23.1 variant, and SARS-CoV-2 S WT with P681R mutation. Data represent the average luciferase activity of cells of four independent experiments (Vero E6 and Vero-TMPRSS2). Error bars represent G standard deviation ( n = 4). Asterisks indicate statistical significance compared to the untreated control. Statistical analysis was performed using an unpaired Student's t test. *, P < 0.1; ****, P < 0.0001. (C) Pseudoparticle infectivity assays in A549-ACE2-TMPRSS2 cells. Cells were infected with MLVpps harboring the SARS-CoV-2 S (WT), SARS-CoV-2 S A.23.1 variant, and SARS-CoV-2 S WT with P681R mutation. Data represent the average luciferase activity of cells of three independent experiments. Error bars represent G standard deviation ( n = 3). Asterisks indicate statistical significance compared to the untreated control. Statistical analysis was performed using an unpaired Student's t test. ****, P < 0.0001.

Article Snippet: SARS-CoV-2 S was detected using a rabbit polyclonal antibody against the S2 domain (cat.: 40590-T62, Sinobiological).

Techniques: Variant Assay, Activation Assay, Western Blot, Produced, Infection, Mutagenesis, Luciferase, Activity Assay, Standard Deviation

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